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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
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Identifying Host Factors Associated with DNA Replicated During Virus Infection
Emigdio D Reyes1,2, Katarzyna Kulej1,2, Neha J Pancholi2,3
1From the ‡Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania.
Molecular & Cellular Proteomics : MCP
|October 4, 2017
Summary
Viruses manipulate host factors to replicate. This study identified cellular processes and nucleolar proteins that aid virus replication, offering insights into virus-host interactions.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Viruses rely on host cell machinery for replication.
- Viral proteins interact with host factors to modulate replication.
- Understanding virus-host interactions is crucial for antiviral strategies.
Purpose of the Study:
- To identify cellular processes manipulated by viral DNA genomes during replication.
- To compare host factor enrichment across different viral infections.
- To uncover specific host factors, like nucleolar proteins, that support viral replication.
Main Methods:
- Proteomic analysis of viral genomes during infection with adenovirus, herpes simplex virus, and vaccinia virus.
- Comparison of host factor enrichment between different viral proteomes.
- Confirmation of host factor association with viral genomes and replication compartments.
Main Results:
- Identified distinct sets of host factors associated with viral genomes during infection.
- Demonstrated that early viral proteins can deactivate specific host factors.
- Uncovered a subgroup of nucleolar proteins that promote adenovirus replication.
Conclusions:
- Viral replication involves extensive manipulation of host cellular processes.
- Nucleolar proteins represent a key resource exploited by viruses for replication.
- The study provides valuable datasets on virus-host interactions at the viral genome level.
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