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Updated: Feb 21, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

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Identifying Host Factors Associated with DNA Replicated During Virus Infection

Emigdio D Reyes1,2, Katarzyna Kulej1,2, Neha J Pancholi2,3

  • 1From the ‡Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania.

Molecular & Cellular Proteomics : MCP
|October 4, 2017
PubMed

Insights

Viruses manipulate host factors to replicate. This study identified cellular processes and nucleolar proteins that aid virus replication, offering insights into virus-host interactions.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Viruses rely on host cell machinery for replication.
  • Viral proteins interact with host factors to modulate replication.
  • Understanding virus-host interactions is crucial for antiviral strategies.

Purpose of the Study:

  • To identify cellular processes manipulated by viral DNA genomes during replication.
  • To compare host factor enrichment across different viral infections.
  • To uncover specific host factors, like nucleolar proteins, that support viral replication.

Main Methods:

  • Proteomic analysis of viral genomes during infection with adenovirus, herpes simplex virus, and vaccinia virus.
  • Comparison of host factor enrichment between different viral proteomes.
  • Confirmation of host factor association with viral genomes and replication compartments.

Main Results:

  • Identified distinct sets of host factors associated with viral genomes during infection.
  • Demonstrated that early viral proteins can deactivate specific host factors.
  • Uncovered a subgroup of nucleolar proteins that promote adenovirus replication.

Conclusions:

  • Viral replication involves extensive manipulation of host cellular processes.
  • Nucleolar proteins represent a key resource exploited by viruses for replication.
  • The study provides valuable datasets on virus-host interactions at the viral genome level.

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