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Updated: Feb 21, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry
Erwin M Berendsen1, Evgeni Levin2, René Braakman1
1Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, Lange Kleiweg 137, 2288 GJ Rijswijk, The Netherlands.
Aim:
Bloodstream infections are a common cause of disease and a fast and accurate identification of the causative agent or agents of bloodstream infections would aid the start of adequate treatment.
Materials & Methods:
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics method was developed for the identification of bacterial species directly from blood cultures that were simulated by inoculating blood culture bottles with single or multiple clinically relevant microorganisms.
Results:
Using LC-MS/MS, the single species were correctly identified in 100% of the blood cultures, whereas for polymicrobial infections, 78% of both species were correctly identified in blood cultures.
Conclusion:
The LC-MS/MS method allows for the identification of the causative agent of positive blood cultures.
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Published on: October 15, 2014
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