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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Sophoraflavenone G Restricts Dengue and Zika Virus Infection via RNA Polymerase Interference
Alexandre Sze1, David Olagnier2, Samar Bel Hadj3
1Department of Medicine, Lady Davis Institute-Jewish General Hospital, McGill University, Montreal, QC, H3T 1E2, Canada. alexandre.sze@mail.mcgill.ca.
Abstract:
Flaviviruses including Zika, Dengue and Hepatitis C virus cause debilitating diseases in humans, and the former are emerging as global health concerns with no antiviral treatments. We investigated Sophora Flavecens, used in Chinese medicine, as a source for antiviral compounds. We isolated Sophoraflavenone G and found that it inhibited Hepatitis C replication, but not Sendai or Vesicular Stomatitis Virus. Pre- and post-infection treatments demonstrated anti-flaviviral activity against Dengue and Zika virus, via viral RNA polymerase inhibition. These data suggest that Sophoraflavenone G represents a promising candidate regarding anti-Flaviviridae research.
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