Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circular and athermal atmospheric CO<sub>2</sub> capture by food waste-derived amyloid sorbents.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Synthesis and pyroelectric response of disperse red 1 functionalized silicones: cyclic monomer, homopolymer, and block copolymer derivatives.

Materials horizons·2026
Same author

Gpnmb defines a phagocytic state of microglia linked to cell death in prion disease mouse model.

Nature communications·2026
Same author

Molecular origins of opalescence and phase separation in mAb formulations and their relation to aggregation.

Communications chemistry·2026
Same author

Photothermal-Activated Antibacterial Amyloid-Polyphenol-Iron Hydrogels for Synergistic Wound Healing.

Advanced healthcare materials·2026
Same author

Resolving liquid-to-glass transitions of water under soft nanoconfinement.

Nature communications·2026

Related Experiment Video

Updated: Feb 21, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.7K

Absolute Quantification of Amyloid Propagons by Digital Microfluidics.

Manuela Pfammatter1, Maria Andreasen2,3, Georg Meisl2

  • 1Institute of Neuropathology, University of Zurich , CH-8091 Zurich, Switzerland.

Analytical Chemistry
|October 4, 2017
PubMed
Summary

This study introduces a digital assay (d-AQuA) to precisely count single protein seeds, called propagons, responsible for amyloid diseases. This breakthrough allows for accurate detection of these disease-driving units in biological samples.

More Related Videos

Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease
09:33

Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

Published on: December 26, 2016

8.5K
Detecting Amyloid-&#946; Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

4.1K

Related Experiment Videos

Last Updated: Feb 21, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.7K
Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease
09:33

Quantitative 3D In Silico Modeling q3DISM of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

Published on: December 26, 2016

8.5K
Detecting Amyloid-&#946; Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

4.1K

Area of Science:

  • Biochemistry
  • Neuroscience
  • Biotechnology

Background:

  • Protein self-replication into amyloid fibrils underlies neurodegenerative diseases.
  • Detecting disease-causing amyloid fibrils is challenging as they resemble non-pathogenic aggregates.
  • Quantifying the replicative capacity of amyloid seeds (propagons) is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To develop a method for the absolute quantification of single protein replicative units (propagons).
  • To validate the digital amyloid quantitative assay (d-AQuA) using insulin as a model protein.

Main Methods:

  • Development of a microfluidics-based digital assay (d-AQuA).
  • Miniaturized propagon-induced amplification chain reactions in picoliter droplets.
  • Stochastic analysis at limiting dilutions for single propagon detection.

Main Results:

  • d-AQuA enables absolute quantification of single propagons at very low concentrations.
  • Quantified propagon numbers correlated with atomic-force microscopy and microplate-based assays.
  • Confirmed the identity of insulin propagons with a subset of morphologically defined protein aggregates.

Conclusions:

  • d-AQuA provides sensitive, precise, and accurate quantification of single protein replicative units.
  • The assay is suitable for biotechnological and medical applications, aiding in neurodegenerative disease research.
  • This technology advances the detection and study of amyloid-related pathologies.