Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

64.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.1K
The DNA Helix01:16

The DNA Helix

158.6K
Overview
158.6K
The DNA Helix01:07

The DNA Helix

30.8K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
30.8K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.6K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.6K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.9K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.9K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.4K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Centroid Regression for Preoperative Risk Assessment of Acute Type A Aortic Dissection Based on Multivariate Clinical Data.

Journal of clinical medicine·2026
Same author

Circulating PEG-indoleamine 2,3-dioxygenase ameliorates diverse inflammatory diseases without toxicity or compromising immunocompetence.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Surgically Diagnosed Diffuse Idiopathic Pulmonary Neuroendocrine Cell Hyperplasia in Asymptomatic Patients.

Surgical case reports·2026
Same author

Reconfigurable Photoelectric Coaxial Fiber-Based Memristors for Neuromorphic Computing.

ACS nano·2026
Same author

TIM3 alleviates microglia-mediated neuroinflammation in neuropathic pain by negatively regulating glycolysis-driven NLRP3 inflammasome activation.

Acta biochimica et biophysica Sinica·2026
Same author

Comparative metabolic safety of efavirenz-, dolutegravir-, and bictegravir-based antiretroviral regimens in HIV-infected adults: Evidence for weight-dependent lipid deterioration.

Journal of infection and public health·2026

Related Experiment Video

Updated: Feb 21, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K

Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA.

Jin Chen1, Qingnan Tang2, Shiwen Guo1

  • 1Mechanobiology Institute, National University of Singapore, 117411, Singapore.

Nucleic Acids Research
|October 4, 2017
PubMed
Summary

Homologous DNA repair involves DNA interactions. Researchers discovered a stable, parallel DNA triplex structure forming spontaneously between extended single-stranded DNA and double-stranded DNA without proteins, potentially aiding DNA repair mechanisms.

More Related Videos

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

21.3K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K

Related Experiment Videos

Last Updated: Feb 21, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

21.3K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • DNA homologous repair is crucial for maintaining genomic stability.
  • This process relies on the interaction between single-stranded DNA (ssDNA) and homologous double-stranded DNA (dsDNA).
  • Understanding the intermediates in this interaction is key to elucidating the repair mechanism.

Purpose of the Study:

  • To investigate the spontaneous formation of DNA structures between ssDNA and dsDNA under force.
  • To characterize the structural and energetic properties of these DNA complexes.
  • To propose a potential role for these structures in recombinase-mediated homologous recombination.

Main Methods:

  • Mechanical force application to induce ssDNA extension.
  • Atomic force microscopy to analyze DNA structure and length.
  • Biophysical measurements to estimate binding energy.

Main Results:

  • A stable, parallel DNA triplex structure spontaneously forms between extended ssDNA and homologous dsDNA in a protein-free environment.
  • The triplex exhibits a contour length similar to B-form dsDNA and remains stable after force removal.
  • The binding energy of the ssDNA-dsDNA triplex is comparable to base-pairing energy in dsDNA.

Conclusions:

  • The spontaneous formation of a stable parallel DNA triplex is demonstrated.
  • This triplex structure may represent a key intermediate in recombinase-catalyzed homologous joint formation during DNA repair.
  • The findings provide insights into the fundamental mechanisms of DNA recombination and repair.