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Genetic diversity of Saccharum complex using ISSR markers
L A R Oliveira1, C A Machado2, M N Cardoso2
1Programa de Pós-Graduação em Agricultura e Biodiversidade, , , Brasil leila.a.resende@gmail.com.
Genetics and Molecular Research : GMR
|October 4, 2017
Summary
Genetic diversity analysis of sugarcane germplasm using inter-simple sequence repeat (ISSR) markers revealed significant variation. This research aids in selecting diverse sugarcane varieties for breeding programs and germplasm exchange.
Area of Science:
- Plant Genetics
- Molecular Biology
- Agronomy
Background:
- Sugarcane (Saccharum sp) cultivation is expanding globally due to increasing demand for raw materials.
- Understanding genetic diversity is crucial for effective germplasm management and breeding strategies.
- The Embrapa Coastal Tablelands Active Germplasm Bank conserves valuable sugarcane genetic resources.
Purpose of the Study:
- To assess the genetic diversity within 26 sugarcane accessions from the Embrapa Coastal Tablelands.
- To utilize inter-simple sequence repeat (ISSR) molecular markers for this genetic diversity analysis.
- To provide data for optimizing breeding and germplasm exchange initiatives.
Main Methods:
- DNA was extracted from 26 sugarcane accessions.
- Inter-simple sequence repeat (ISSR) molecular markers were employed.
- Genetic similarity and population structure were analyzed using UPGMA clustering.
Main Results:
- Sixteen ISSR primers generated 87 polymorphic DNA fragments, indicating 91.13% polymorphism.
- Genetic similarity among accessions ranged from 0.22 to 0.87.
- Four distinct genetic clusters were identified, with specific accessions identified as genetically close or distant.
Conclusions:
- The study successfully characterized the genetic diversity of the analyzed sugarcane germplasm.
- Findings can guide the selection of parental lines for hybridization and facilitate informed germplasm exchange.
- This genetic information is vital for enhancing sugarcane breeding programs and conservation efforts.
