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Allele frequencies in Azuay Population in Ecuador
P P Orellana1, C F Andrade2, C L Arciniegas3
1Academic Unit of Health and Welfare, Odontology Career, Laboratory of Molecular Biology and Genetics, , , Ecuador porellana@ucacue.edu.ec.
Genetics and Molecular Research : GMR
|October 4, 2017
Summary
Forensic genetics in Ecuador analyzed DNA markers from 182 individuals for paternity testing. High polymorphic markers like SE33 offer superior discrimination power for genetic identification.
Area of Science:
- Forensic Genetics
- Molecular Biology
- Population Genetics
Background:
- Paternity testing relies on analyzing highly variable DNA markers.
- Establishing population-specific genetic data is crucial for accurate forensic analysis.
- Previous genetic marker studies in Ecuador are limited.
Purpose of the Study:
- To determine allele frequencies and genetic diversity of 16 short tandem repeat (STR) markers in the Azuay province of Ecuador.
- To evaluate the forensic suitability of these STR markers for paternity testing and individual identification.
- To assess the genetic variation within the studied population.
Main Methods:
- DNA was extracted from blood samples of 182 unrelated individuals.
- Sixteen STR loci (D22S1045, D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D1S1656, D12S391, D10S1248, D2S441, SE33) were amplified using polymerase chain reaction (PCR).
- Electrophoresis was used for DNA fragment separation, followed by allele frequency estimation and heterozygosity calculations. Hardy-Weinberg equilibrium was assessed.
Main Results:
- Allele frequencies were estimated for all analyzed STR markers.
- Markers with the highest polymorphic information content (PIC) and greatest discriminatory power were identified, including D21S11, D16S539, D2S1338, D19S433, D18S51, FGA, D1S1656, and D12S391.
- The SE33 marker exhibited the highest PIC value, indicating exceptional polymorphic characteristics and high discrimination potential.
Conclusions:
- The studied STR markers are highly polymorphic and suitable for paternity testing and forensic applications in the Azuay province.
- Population-specific allele frequency data generated in this study will enhance the accuracy of genetic identification in Ecuador.
- The SE33 marker is particularly valuable for forensic casework due to its high discriminatory power.
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