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Quantitation of Minor Components in Mango Juice with Band-Selective Excitation NMR Spectroscopy
Shoraku Ryu1, Masanori Koda1, Takuya Miyakawa1
1Laboratory of Basic Science on Healthy Longevity, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Abstract:
1H NMR-based metabolic analysis of foods has been widely applied. However, dynamic range problems frequently impede its application because foodstuffs are composed of various organic compounds in a wide range of concentrations. Band-selective excitation 1H NMR spectroscopy has been found to be a useful tool for observing the minor components in foods. Because quantitative information is important for metabolic analysis of foods and complete metabolome data, quantitation with the band-selective excitation 1H NMR method was carefully investigated in the present study. As a result, the concentrations of minor components in mango juice of the "Carabao" cultivar were successfully quantitated by band-selective excitation 1H NMR using standard curves that exhibited good linearity. The band-selective excitation 1H NMR technique was therefore effective for determining the concentrations of minor components in foods.
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