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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Updated: Feb 21, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Reprogramming Mouse Fibroblasts with piggyBac Transposons.

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy

    Cold Spring Harbor Protocols
    |October 5, 2017
    PubMed
    Summary
    This summary is machine-generated.

    Scientists reprogramed somatic cells into induced pluripotent stem (iPS) cells using four genes. This protocol details a simple method for generating iPS cells from mouse fibroblasts efficiently.

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    Area of Science:

    • Cell Biology
    • Stem Cell Research
    • Genetics

    Background:

    • Somatic cells can be reprogrammed into pluripotent stem cells.
    • Induced pluripotent stem (iPS) cells share characteristics with embryonic stem (ES) cells.
    • The Yamanaka factors (four specific genes) are key to cellular reprogramming.

    Purpose of the Study:

    • To describe a simple protocol for generating iPS cells from mouse fibroblasts.
    • To utilize an efficient transposon-mediated transfection method.
    • To employ a tetracycline-inducible system for controlled gene expression.

    Main Methods:

    • Transfection of mouse fibroblasts with reprogramming factors.
    • Use of a transposon-mediated delivery system.
    • Application of a tetracycline-inducible system to regulate gene expression.

    Main Results:

    • Successful generation of induced pluripotent stem (iPS) cells from mouse fibroblasts.
    • Demonstration of an efficient reprogramming method.
    • Validation of the tetracycline-inducible system for controlling Yamanaka factor expression.

    Conclusions:

    • A simplified and efficient protocol for generating iPS cells has been established.
    • This method facilitates the study of cellular reprogramming.
    • The technique offers a controlled approach to producing iPS cells for research.