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Related Experiment Video

Updated: Feb 21, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Published on: September 20, 2019

Transcriptional Output Transiently Spikes Upon Mitotic Exit.

Viola Vaňková Hausnerová^1,2, Christian Lanctôt³

¹BIOCEV and Department of Cell Biology, Faculty of Science, Charles University, Vestec, 252 50, Czech Republic.

Scientific Reports

|October 5, 2017

Summary

Gene activity occurs in bursts. Researchers found transient transcriptional spikes after cell division (mitotic exit) in human cells, suggesting unique regulation during this phase.

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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Gene activity is pulsatile, characterized by transcriptional bursts.
The frequency and amplitude of these bursts can be externally regulated.
Understanding cell cycle regulation of gene expression is crucial.

Purpose of the Study:

To investigate if transcriptional burst parameters (frequency, amplitude) change throughout the cell cycle.
To identify the timing and characteristics of gene activity changes post-mitotic exit.
To explore the regulatory mechanisms underlying post-mitotic gene expression.

Main Methods:

Single molecule RNA FISH technique to visualize and quantify transcripts.
Immuno-RNA FISH combined with cell growth recording.
Analysis of transcriptional output in three human cell lines.

Main Results:

Evidence of transient transcriptional spikes immediately following mitotic exit in all tested cell lines.
These spikes were short-lived, subsiding before cytokinesis completion.
The increase in transcriptional output resulted from more active alleles and/or more transcripts per active allele, indicating changes in burst fraction and amplitude.
Distinct regulatory mechanisms appear active post-mitotic exit compared to interphase.

Conclusions:

Gene expression exhibits transient increases immediately after cell division (mitotic exit).
Both the frequency (burst fraction) and intensity (amplitude) of transcriptional bursts are modulated post-mitosis.
Transcriptional spikes may be linked to chromatin decondensation, influencing transcriptional regulator dynamics.