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Variation in indirect cell-mediated lympholysis.

N M Ennulat1, D Tice, D Carpenter

  • 1Department of Surgery, SUNY Health Science Center, Syracuse 13210.

Journal of Clinical & Laboratory Immunology
|February 1, 1988
PubMed
Summary
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The indirect cell-mediated lympholysis (ICML) assay is reproducible for assessing allograft rejection. Cytotoxicity magnitude depends on activated target cells and T-lymphocyte proportions during effector cell generation.

Area of Science:

  • Immunology
  • Transplantation immunology
  • Cellular immunology

Background:

  • The indirect cell-mediated lympholysis (ICML) assay is an in vitro correlate of allograft rejection.
  • Inconsistencies in micro-ICML assay results have been observed, questioning its reliability.

Purpose of the Study:

  • To investigate the extent and reasons for inconsistency in the micro-ICML assay.
  • To determine the reproducibility of the micro-ICML assay and identify factors influencing its variability.

Main Methods:

  • Duplicate micro-ICML assays were performed to assess method variation.
  • Cytotoxicity was measured over time to evaluate temporal variation.
  • 51Cr release from target cells was analyzed to determine its contribution to variation.
  • Cell surface antigen frequencies (DR, TAC, transferrin, Leu 2, Leu 3) were measured alongside ICML.

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Main Results:

  • Method variation and variation over time were not statistically significant.
  • The contribution of 51Cr release from target cells to overall variation was minimal (6.33%).
  • Reproducibility was confirmed, with lysis magnitude correlating with activated target cells and specific T-lymphocyte proportions.

Conclusions:

  • The micro-ICML assay is a reproducible method for assessing allograft rejection.
  • Activated target cells and specific T-lymphocyte subset ratios are critical for reliable ICML results.