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This study introduces a novel dual cure polymer for microfluidic gaskets, enabling room-temperature bonding to protein surfaces. This method maintains biological activity and improves throughput for miniaturized biomolecular assays.

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Area of Science:

  • Biomolecular assays
  • Microfluidics
  • Polymer science

Background:

  • Improving sensitivity and throughput in miniaturized biomolecular assays requires efficient biomolecule transport.
  • Integrating microfluidics necessitates bonding gaskets to assay surfaces without compromising biological activity.

Purpose of the Study:

  • To address the challenge of sealing microfluidic gaskets to protein surfaces while maintaining biological activity.
  • To develop a novel polymer resin for microfluidic gasket formation and room-temperature bonding.

Main Methods:

  • Development of a novel dual cure polymer resin for microfluidic gaskets.
  • Room-temperature bonding of gaskets to various substrates using UVA light.
  • Characterization of polymer properties (stiffness, biomolecule absorption, leaching) and bonding performance via FT-IR and multiplexed immunoassays.

Main Results:

  • The novel polymer allows room-temperature bonding of microfluidic gaskets to protein surfaces using UVA light.
  • The cured gaskets exhibit high stiffness, minimal biomolecule absorption, and no significant monomer leaching.
  • Demonstrated maintained biological activity and high repeatability in multiplexed immunoassays on protein arrays.

Conclusions:

  • A novel dual cure polymer enables effective room-temperature bonding of microfluidic gaskets to biofunctionalized surfaces.
  • This approach preserves biological activity and offers potential for increased sample throughput and reduced manufacturing costs in miniaturized biomolecular systems.