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Recombinant spider silk-based bioinks.

Elise DeSimone1, Kristin Schacht, Alexandra Pellert

  • 1Lehrstuhl Biomaterialien, Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayreuther Zentrum für Bio-Makromoleküle (bio-mac), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Bayerisches Polymerinstitut (BPI) Universitätsstraße 30, Universität Bayreuth, Bayreuth D-95447, Germany.

Biofabrication
|October 5, 2017
PubMed
Summary
This summary is machine-generated.

Recombinant spider silk proteins show promise as bioinks for 3D cell culture. While initial hydrogels supported cell growth, printing inhibited proliferation; adding gelatin improved printed scaffold resolution and shows potential for future bioink development.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biotechnology

Background:

  • Bioinks are crucial for 3D cell culture but are in early development.
  • Recombinant spider silk proteins offer biocompatibility and modifiability for bioink applications.
  • Current research focuses on printer design, scaffold materials, and bioink modification.

Purpose of the Study:

  • To prepare and characterize thermally gelled hydrogels from recombinant spider silk protein for bioink applications.
  • To evaluate the in vitro performance of these bioinks before and after 3D printing.
  • To investigate the effect of gelatin as an additive to improve printed hydrogel properties.

Main Methods:

  • Preparation of thermally gelled hydrogels using recombinant spider silk protein.
  • Encapsulation of BALB/3T3 mouse fibroblast cell line within the hydrogels.
  • In vitro evaluation of cell viability, spreading, and proliferation before and after 3D printing.
  • Assessment of hydrogel properties and printed scaffold resolution with and without gelatin additive.

Main Results:

  • Unprinted spider silk-based bioinks supported cell spreading and proliferation.
  • 3D printing of the bioinks into scaffolds prohibited cell proliferation.
  • Addition of gelatin as a plasticizer improved the resolution of printed hydrogel strands.

Conclusions:

  • Recombinant spider silk protein hydrogels demonstrate potential as a bioink material.
  • Further development is needed to overcome proliferation inhibition in printed scaffolds.
  • Gelatin shows promise as an additive for improving the printability and resolution of spider silk-based bioinks.