Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.5K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.5K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.3K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.3K
Autophagy01:27

Autophagy

5.9K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.9K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

5.8K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
5.8K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.2K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.2K
Mitochondria01:37

Mitochondria

20.9K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aging promotes inflammation and steatosis in alcohol-associated liver disease in mice.

bioRxiv : the preprint server for biology·2026
Same author

Myofibroblast- specific autophagy drives cyst growth in autosomal dominant polycystic kidney disease.

Cell communication and signaling : CCS·2026
Same author

Myofibroblast- specific autophagy drives cyst growth in autosomal dominant polycystic kidney disease.

bioRxiv : the preprint server for biology·2026
Same author

Musculoskeletal pain in medical laboratory personnel: a cross-sectional study.

American journal of clinical pathology·2026
Same author

Megamitochondria in alcohol-associated liver disease and cancer: a friend or foe?

eGastroenterology·2026
Same author

Role of myeloid cell transcription factor EB in alcohol-induced liver injury in mice.

Liver research (Beijing, China)·2026

Related Experiment Video

Updated: Feb 21, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

6.3K

Mechanisms, pathophysiological roles and methods for analyzing mitophagy - recent insights.

Jessica A Williams1, Wen-Xing Ding2

  • 1Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160, USA.

Biological Chemistry
|October 5, 2017
PubMed
Summary

This review updates the rapid advances in mitophagy, a key cellular process. It covers new molecular mechanisms, pathophysiological roles, and methods for analyzing mitophagy in various organisms and tissues.

Keywords:
Mito-QCParkinautophagymitoTimermitophagymt-Keima

More Related Videos

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

18.4K
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

15.8K

Related Experiment Videos

Last Updated: Feb 21, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

6.3K
In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

18.4K
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

15.8K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Molecular Biology

Background:

  • The field of mitophagy has expanded significantly since 2012.
  • Numerous new molecular mechanisms and tools for studying mitophagy have emerged.

Purpose of the Study:

  • To provide an updated review of mitophagy.
  • Focus on basic molecular mechanisms, pathophysiological roles, and analytical methods.

Main Methods:

  • Literature review of recent advancements in mitophagy research.
  • Focus on molecular mechanisms and analytical techniques.

Main Results:

  • Significant progress in understanding mitophagy regulation and function.
  • Development of novel tools for mitophagy monitoring in cells and in vivo.

Conclusions:

  • Mitophagy research has advanced considerably, offering new insights into cellular processes.
  • Current methods for mitophagy analysis have both strengths and limitations that warrant consideration.