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Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Feb 21, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

On site DNA barcoding by nanopore sequencing.

Michele Menegon¹, Chiara Cantaloni^2,3, Ana Rodriguez-Prieto¹

¹Tropical Biodiversity section, Science Museum of Trento, Trento, Italy.

|October 5, 2017

Summary

This summary is machine-generated.

A portable DNA sequencing lab enables field-based species identification using DNA barcoding. This technology overcomes limitations of cost and infrastructure, advancing biodiversity research in remote locations.

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Last Updated: Feb 21, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Genomics
Biodiversity Research
Molecular Ecology

Background:

Genomic tools are crucial for biodiversity research, enabling detailed understanding of Earth's biological diversity.
DNA barcoding is a powerful method for species identification, but its application is limited by high costs and lack of facilities in resource-limited regions.

Purpose of the Study:

To develop and evaluate a portable DNA sequencing laboratory for field-based DNA barcoding.
To assess the feasibility of using portable sequencing technology in remote, challenging environments for biodiversity assessment.

Main Methods:

Development of a portable laboratory using the Oxford Nanopore MinION sequencer and complementary equipment for remote use.
Testing the portable system for DNA barcoding in a simulated tropical environment and a remote Tanzanian rainforest.
Implementation of a specialized data analysis pipeline to manage sequencing data and identify species.

Main Results:

Accurate species identification of vertebrates (amphibians, reptiles, mammals) was achieved despite the MinION's error rate.
Successful in situ DNA barcoding of a wild frog in the field demonstrated real-time species identification capabilities.
The portable laboratory performed effectively in a remote rainforest environment lacking electricity.

Conclusions:

Portable DNA sequencing offers a viable solution for DNA barcoding in remote areas lacking traditional laboratory infrastructure.
This approach enhances opportunities for real-time, on-site biodiversity assessment and understanding.
The developed system has the potential to significantly advance biodiversity research globally, especially in under-equipped regions.