Related Experiment Video
Updated: Feb 21, 2026
09:48
Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
Published on: May 4, 2015
14.3K
Fast hyperbaric decompression after heliox saturation altered the brain proteome in rats
Alvhild Alette Bjørkum1, Eystein Oveland2,3, Linda Stuhr4
1Department of Biomedical Laboratory Sciences and Chemical Engineering, Western Norway University of Applied Sciences, Bergen, Norway.
Plos One
|October 5, 2017
Summary
Fast decompression after heliox saturation diving in rats altered the brain proteome, impacting synaptic vesicle function and protein homeostasis. This research offers insights into neurological symptoms of decompression sickness.
Area of Science:
- Neuroscience
- Proteomics
- Diving Physiology
Background:
- Decompression sickness (DCS) poses risks to divers.
- Understanding DCS's neurological effects is crucial for improving diving safety.
Purpose of the Study:
- To investigate the impact of rapid heliox decompression on the rat brain proteome.
- To identify specific proteins and pathways affected by fast decompression.
Main Methods:
- Heliox saturation diving model in rats.
- Comparison of fast decompression (1 bar/20 s) versus slow decompression (1 bar/10 min).
- Quantitative proteomic analysis using iontrap and orbitrap LC-MS.
Main Results:
- Significant alterations in brain proteome identified, with 56 proteins (iontrap) and 128 proteins (orbitrap) regulated.
- Enrichment analysis revealed affected networks: synaptic vesicle fusion/recycling and translation initiation.
- Key proteins like ribosomal proteins, hippocalcin-like protein 4, and proteasome subunit beta type-7 were upregulated; heat shock protein 105 kDa, Rho-associated protein kinase 2, and Dynamin-1 were downregulated.
Conclusions:
- Fast decompression disrupts brain proteome, affecting protein homeostasis and synaptic vesicle function.
- Potential implications for neurological disorders due to altered synaptic mechanisms.
- Findings contribute to a better understanding of DCS pathophysiology.
