Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.7K
5.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.7K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.7K
Master Transcription Regulators02:23

Master Transcription Regulators

7.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.8K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diurnal regulation of SDG2 and JMJ14 by circadian clock oscillators orchestrates histone modification rhythms in Arabidopsis.

Genome biology·2019
Same author

The relationship between knee joint angle and knee flexor and extensor muscle strength.

Journal of physical therapy science·2017
Same author

DNA context represents transcription regulation of the gene in mouse embryonic stem cells.

Scientific reports·2016
Same author

Erratum to: Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3.

Genome biology·2016
Same author

Effect of pelvic floor muscle exercises on pulmonary function.

Journal of physical therapy science·2015
Same author

Effects of cervical self-stretching on slow vital capacity.

Journal of physical therapy science·2015

Related Experiment Video

Updated: Feb 21, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.7K

Gene-regulatory interactions in embryonic stem cells represent cell-type specific gene regulatory programs.

Misook Ha1, Soondo Hong2

  • 1Samsung Advanced Institute of Technology, Samsung Electronics Corporation, Suwon 443-803, Korea.

Nucleic Acids Research
|October 5, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to predict how embryonic stem cells (ESCs) differentiate. This approach decodes gene regulatory programs within ESCs to understand cell specialization and developmental changes.

More Related Videos

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.7K

Related Experiment Videos

Last Updated: Feb 21, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.7K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.7K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Systems Biology

Background:

  • Pluripotency in embryonic stem cells (ESCs) is regulated by specific gene regulators.
  • Understanding how ESCs prepare for differentiation and predicting molecular changes is crucial but not well understood.

Purpose of the Study:

  • To develop a novel computational approach for inferring cell-type specific gene regulatory programs from ESCs.
  • To provide methods for the prognosis of molecular changes during ESC differentiation into specific organs.

Main Methods:

  • Developed a method to infer gene regulatory programs by analyzing position-specific combinatorial patterns of regulators in ESCs.
  • Mapped these patterns to predict cell-type specific gene regulations.
  • Validated the approach using RNA-seq and microarray data from neuronal progenitor cells, adult liver cells, and ESCs.

Main Results:

  • The proposed method successfully recapitulated existing gene expression data for differentiated cell types.
  • Demonstrated that collective functions of diverse gene regulators in ESCs define distinct regulatory programs in specialized cells.
  • Showcased the ability to infer cell-type specific gene regulatory mechanisms from ESCs.

Conclusions:

  • The new approach expands the understanding of differential gene regulatory information encoded within ESC regulatory networks.
  • Highlights the potential of ESCs to hold latent gene regulatory programs for various cell types.
  • Provides a framework for studying developmental trajectories and cell fate determination.