Monoclonal antibodies to monkey pox virus: preparation and application

Two fusion experiments with NSO myeloma cells yielded 30 hybrid cell lines which continuously and actively secreted monoclonal antibodies (MoAb) to monkey pox virus. Eight lines appeared to produce antibodies to specific antigenic determinants. The isotype and serologic reactivity of MoAb to monkey pox virus was characterized and the karyotype of several hybridomas determined. An enzyme labelled conjugate has been prepared from MoAb selectively reacting with monkey pox virus. This conjugate allowed the identification of monkey pox virus in the materials from patients. It also helped to determine that an orthopoxvirus isolated from wild squirrel in the Republic of Zaire was monkey pox virus.