Related Experiment Video

Updated: Feb 21, 2026

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

20.0K

Low-dose photon irradiation induces invasiveness through the SDF-1α/CXCR4 pathway in malignant mesothelioma cells

Yoshikane Yamauchi1, Seyer Safi1, Lena Orschiedt2,3

  • 1Department of Thoracic Surgery, Thorax Clinic, Heidelberg University, Heidelberg, Germany.

Oncotarget
|October 6, 2017
PubMed
Abstract

Insights

Low-dose photon radiation increases malignant pleural mesothelioma cell migration via the SDF-1α/CXCR4 pathway. Carbon ions did not promote migration, suggesting alternative radiotherapy strategies to reduce recurrence risk.

Area of Science:

  • Oncology
  • Radiotherapy
  • Cell Biology

Background:

  • Low-dose photon irradiation is suspected to increase local recurrence and systemic dissemination risks.
  • Malignant pleural mesothelioma (MPM) is a challenging cancer with limited treatment options.

Purpose of the Study:

  • To investigate the effect of low-dose photon irradiation on MPM cell motility.
  • To elucidate the underlying molecular mechanisms of radiation-induced changes in MPM cell behavior.

Main Methods:

  • Clonogenic survival and migration assays were performed on MPM cell lines (H28, H226).
  • Cells were exposed to photon and carbon ion irradiation at various doses.
  • Expression of CXCR4, SDF-1α, and integrins was analyzed using FACS, ELISA, and Western blot.
  • Apoptosis was assessed via Annexin-V staining.

Main Results:

  • Low-dose photon irradiation (1-2 Gy) suppressed clonogenicity but promoted MPM cell migration.
  • MPM cell migration was enhanced via the stromal cell-derived factor 1α (SDF-1α)/C-X-C chemokine receptor type 4 (CXCR4) pathway.
  • The CXCR4 antagonist AMD3100 inhibited photon irradiation-induced hypermigration.
  • Carbon ion irradiation (0.3-1 Gy) suppressed clonogenicity without promoting migration.

Conclusions:

  • Photon irradiation at low doses can enhance MPM cell migration through the SDF-1α/CXCR4 pathway.
  • Carbon ion irradiation may offer a safer alternative by not promoting cell migration.
  • Co-administration of photon irradiation with CXCR4 antagonists or using carbon ions could reduce locoregional recurrence risk in MPM radiotherapy.
Keywords:
CXCR4SDF-1αcarbon ion irradiationmesotheliomaphoton irradiation

More Related Videos

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model09:31

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Published on: October 2, 2015

9.7K
Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA04:12

Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA

Published on: December 19, 2019

15.4K

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K