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The long non-coding RNA NONHSAT062994 inhibits colorectal cancer by inactivating Akt signaling

Xiao-Shun He1, Ling-Chuan Guo1, Ming-Zhan Du1

  • 1Department of Pathology, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.

Oncotarget
|October 6, 2017
PubMed

Insights

Long noncoding RNA (lncRNA) NONHSAT062994 acts as a tumor suppressor in colorectal cancer (CRC). Its downregulation promotes CRC growth by activating Akt signaling, suggesting it as a potential biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant long noncoding RNA (lncRNA) expression is linked to cancer development.
  • The role of NONHSAT062994 in colorectal cancer (CRC) pathogenesis remains unclear.

Purpose of the Study:

  • To investigate the clinical significance and functional mechanism of NONHSAT062994 in CRC.
  • To determine if NONHSAT062994 can serve as a prognostic biomarker or therapeutic target for CRC.

Main Methods:

  • Quantitative real-time PCR to assess NONHSAT062994 expression in CRC tissues and cell lines.
  • In vitro and in vivo assays to evaluate the effect of NONHSAT062994 manipulation on CRC cell growth.
  • Western blot analysis to examine Akt signaling pathway activation and downstream targets.

Main Results:

  • NONHSAT062994 was significantly downregulated in CRC tissues and cell lines.
  • Lower NONHSAT062994 expression correlated with larger tumor size and poorer overall survival in CRC patients.
  • NONHSAT062994 overexpression inhibited CRC cell proliferation, while its knockdown enhanced it, both in vitro and in vivo.
  • NONHSAT062994 suppressed CRC cell growth by inactivating the Akt signaling pathway.
  • NONHSAT062994 expression negatively correlated with Akt downstream targets c-Myc and Cyclin D1 in clinical samples.

Conclusions:

  • NONHSAT062994 functions as a tumor suppressor in CRC development by inhibiting the Akt signaling pathway.
  • NONHSAT062994 represents a potential prognostic biomarker and therapeutic target for colorectal cancer.
Keywords:
Akt signalingLncRNA NONHSAT062994colorectal cancergrowth

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