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Related Experiment Videos

A modified m-CP medium for enumerating Clostridium perfringens from water samples.

R Armon1, P Payment

  • 1Centre de recherche en virologie, Institut Armand-Frappier, Ville de Laval, Qué, Canada.

Canadian Journal of Microbiology
|January 1, 1988
PubMed
Summary

Reducing indoxyl beta-D-glucoside in m-CP medium for Clostridium perfringens isolation to 60 mg/L significantly cuts costs. This lower concentration maintains the medium's sensitivity for detecting the bacteria in water samples.

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Area of Science:

  • Microbiology
  • Water Quality Analysis
  • Culture Media Development

Background:

  • Clostridium perfringens is a significant waterborne pathogen.
  • The m-CP medium is a specialized culture medium for isolating Clostridium perfringens.
  • Indoxyl beta-D-glucoside is a key, but costly, component of m-CP medium.

Purpose of the Study:

  • To evaluate the impact of reduced indoxyl beta-D-glucoside concentration on m-CP medium performance.
  • To determine if cost savings can be achieved without compromising Clostridium perfringens detection.
  • To optimize the formulation of m-CP medium for water sample analysis.

Main Methods:

  • Testing m-CP medium with varying concentrations of indoxyl beta-D-glucoside (0, 60, and 600 mg/L).
  • Assessing the sensitivity of the medium for Clostridium perfringens isolation from water samples.

Related Experiment Videos

  • Comparing the cost-effectiveness of different medium formulations.

    • Main Results:

    • A reduced concentration of 60 mg/L indoxyl beta-D-glucoside (1/10th of the original) did not affect the sensitivity of m-CP medium.
    • The lower concentration of indoxyl beta-D-glucoside significantly reduced the overall cost of the medium.
    • The medium remained effective for isolating Clostridium perfringens.

    Conclusions:

    • The cost of m-CP medium can be substantially reduced by lowering the indoxyl beta-D-glucoside concentration.
    • Optimized m-CP medium formulation maintains diagnostic sensitivity for Clostridium perfringens.
    • This cost-effective approach enhances the practicality of Clostridium perfringens surveillance in water quality testing.