The modulation of excitatory amino acid responses by serotonin in the cat neocortex in vitro

1. The electrophysiological actions of excitatory amino acids and serotonin were investigated in slices from cat neocortex in vitro. Intracellular recordings were obtained from neurons (mainly in layer V) and the drugs applied extracellularly to the same neurons by microiontophoresis. 2. Serotonin, and to some extent noradrenaline, facilitated the excitatory actions of N-methyl-D-aspartate (NMDA), glutamate, and quisqualate but caused no changes in the passive neuronal membrane properties when presented alone. Serotonin had no effect on evoked excitatory postsynaptic potentials (EPSPs) or spike afterhyperpolarizations. 3. The facilitatory effect of serotonin on the responses to NMDA was observed with both somatic and dendritic applications. It persisted during Mg2+ depletion and in the presence of tetrodotoxin and tetraethylammonium. The effect was attenuated by the serotonin antagonist cinanserin but not by methysergide. A possible underlying receptor modulation is discussed.