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Effect of Crude Venom of Odonthobuthus doriae Scorpion in Cell Culture using Ion Channel Modulators

Sainaz Ghasemi1, Amir Ahmad Salarian2, Abbas Zare Mirakabadi3

  • 1Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Iranian Journal of Pharmaceutical Research : IJPR
|October 6, 2017
PubMed

Insights

The yellow scorpion (Odonthobuthus doriae) venom is toxic to cancerous nerve cells. Its cytotoxic effects are mediated through sodium (Na) channels, as shown by experiments using ion channel blockers.

Area of Science:

  • Toxicology
  • Neuroscience
  • Cell Biology

Background:

  • Scorpion venom poses significant health risks, with increasing research into its cytotoxic mechanisms.
  • Odonthobuthus doriae, the yellow scorpion, is a notable threat in Iran due to its neurotoxicity.
  • Previous studies indicate yellow scorpion venom affects ion channels in isolated organs and cell lines.

Purpose of the Study:

  • To investigate the cytotoxic effects of Odonthobuthus doriae crude venom on the 1321N1 cell line.
  • To determine the role of specific ion channels in the venom's cytotoxicity.

Main Methods:

  • Utilized the MTT assay to assess cell viability.
  • Exposed 1321N1 cells (cancerous nerve cells) to crude O. doriae venom.
  • Investigated the venom's effects in the presence of ion channel blockers: Ouabain (Na+ channel blocker), Nifedipine (Ca2+ channel blocker), and TEA (K+ channel blocker).

Main Results:

  • Odonthobuthus doriae crude venom demonstrated significant cytotoxic effects on the 1321N1 cell line.
  • The cytotoxic mechanism was primarily mediated through sodium (Na+) channels.

Conclusions:

  • The study confirms the cytotoxic potential of O. doriae venom on cancerous nerve cells.
  • Sodium channels are key targets for the venom's toxic action, providing insights into its neurotoxic effects.
Keywords:
1321N1Channel blockerOdonthobuthus doriaeScorpioncell culture

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