Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

HuBIE: The human blood immunome encyclopedia of TCRs and BCRs in bloodstream infections and cancer.

Frontiers in immunology·2026

Same author

Gel Shrinkage in Discontinuous Electrophoresis: How to Stabilize the Electrolyte Boundary in Epitachophoresis - Part 2 - Nongel Solid Support.

ACS omega·2026

Same author

Gel Shrinkage in Discontinuous Electrophoresis: How to Stabilize the Electrolyte Boundary in EpitachophoresisPart 1Gel Selection.

ACS omega·2026

Same author

HuBIE: The Human Blood Immunome Encyclopedia Of TCRs and BCRs in Bloodstream Infections and Cancer.

bioRxiv : the preprint server for biology·2025

Same author

Editorial for New Frontiers in Electrophoresis.

Talanta·2025

Same author

Epitope Mapping Using Yeast Display and Next Generation Sequencing.

Methods in molecular biology (Clifton, N.J.)·2025

Search research articles

Related Experiment Video

Updated: Feb 21, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Recent progress in nucleic acids isotachophoresis.

Vladimíra Datinská^1,2, Ivona Voráčová¹, Ulrich Schlecht³

¹Czech Academy of Sciences, Institute of Analytical Chemistry, Brno, Czech Republic.

Journal of Separation Science

|October 6, 2017

Summary

This summary is machine-generated.

Isotachophoresis offers a quantitative method for nucleic acid extraction and purification from complex samples. This review covers 2014-2017 advancements in isotachophoresis for nucleic acid concentration, overcoming limitations of traditional techniques.

Keywords:

isotachophoresis nucleic acids sample preparation

More Related Videos

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

DNA Stable-Isotope Probing DNA-SIP

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

Related Experiment Videos

Last Updated: Feb 21, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

DNA Stable-Isotope Probing DNA-SIP

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

Area of Science:

Biochemistry
Analytical Chemistry
Molecular Biology

Background:

Nucleic acid isolation is often hindered by complex sample matrices like blood and forensic samples.
Traditional nucleic acid extraction methods have limitations in quantitative accuracy.
Isotachophoresis (ITP) presents a quantitative alternative for nucleic acid purification.

Purpose of the Study:

To review progress in nucleic acid extraction and sample preparation using isotachophoresis between 2014-2017.
To highlight the advantages of ITP for nucleic acid concentration.
To provide a brief overview of ITP principles for nucleic acid purification.

Main Methods:

Review of scientific literature on isotachophoresis applications for nucleic acid purification from 2014-2017.
Description of the fundamental principles of isotachophoresis.
Focus on advancements in sample preparation and nucleic acid concentration techniques.

Main Results:

Isotachophoresis demonstrates a theoretically unlimited concentration factor for nucleic acids.
ITP provides quantitative results, unlike many traditional extraction methods.
Recent developments have enhanced ITP's utility in complex biological and forensic samples.

Conclusions:

Isotachophoresis is a promising technique for quantitative nucleic acid isolation from challenging samples.
Continued research and development in ITP can overcome limitations of conventional methods.
ITP offers significant potential for applications in molecular biology, forensics, and diagnostics.