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Physiopathological roles of spontaneously released outer membrane vesicles of Bordetella pertussis

Gianmarco Gasperini1, Vanessa Arato1, Mariagrazia Pizza1

  • 1GSK Vaccines, Via Fiorentina 1, 53100, Siena, Italy.

Future Microbiology
|October 6, 2017
PubMed
Abstract

Insights

Bordetella pertussis releases outer membrane vesicles (OMV) that deliver iron and contribute to host cell intoxication during airway infections. These OMV play diverse roles in pertussis pathogenesis.

Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogenesis

Background:

  • Bordetella pertussis releases outer membrane vesicles (OMV).
  • The biological role of OMV during initial B. pertussis airway infection is largely unknown.

Purpose of the Study:

  • To investigate the biological role of OMV during B. pertussis infection of human ciliated airway cells.
  • To determine the host-cell interaction, regulation, and function of B. pertussis OMV.

Main Methods:

  • Utilized a human ciliated-airway cell model to study OMV release and interaction.
  • Purified OMV and assessed their binding, uptake, and iron-delivery capabilities.
  • Investigated the Bvg-regulation of OMV-host cell interactions.

Main Results:

  • OMV are released by B. pertussis and interact with host cells.
  • OMV binding and uptake are regulated by the Bvg locus.
  • Pertussis toxin associated with OMV contributes to host cell intoxication.
  • OMV function as iron-delivery systems, supporting B. pertussis growth under iron-limiting conditions.

Conclusions:

  • OMV play multifaceted roles in B. pertussis pathogenesis.
  • Further investigation into OMV functions is warranted.
Keywords:
Bordetella pertussisepithelial cellsintoxicationiron deliveryouter membrane vesicles

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