Related Experiment Video
Updated: Feb 21, 2026
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
Published on: September 13, 2017
Cell division cycle 20 promotes cell proliferation and invasion and inhibits apoptosis in osteosarcoma cells
Guanning Shang1, Xu Ma1, Gang Lv1
1a Department of Orthopaedics , The First Affiliated Hospital , China Medical University , Shenyang , Liaoning Province , PR China.
Abstract:
Cdc20 (cell division cycle 20 homologue) has been reported to exhibit an oncogenic role in human tumorigenesis. However, the function of Cdc20 in osteosarcoma (OS) has not been investigated. In the current study, we aim to explore the role of Cdc20 in human OS cells. Multiple approaches were used to measure cell growth, apoptosis, cell cycle, migration and invasion in OS cells after depletion of Cdc20 or overexpression of Cdc20. We found that down-regulation of Cdc20 inhibited cell growth, induced apoptosis and triggered cell cycle arrest in OS cells. Moreover, Cdc20 down-regulation let to inhibition of cell migration and invasion in OS cells. Consistently, overexpression of Cdc20 in OS cells promoted cell growth, inhibited apoptosis, enhanced cell migration and invasion. Mechanistically, our Western blotting results showed that overexpression of Cdc20 reduced the expression of Bim and p21, whereas depletion of Cdc20 upregulated Bim and p21 levels in OS cells. Altogether, our findings demonstrated that Cdc20 exerts its oncogenic role partly due to regulation of Bim and p21 in OS cells, suggesting that targeting Cdc20 could be useful for the treatment of OS.
Insights
Cell division cycle 20 homologue (Cdc20) drives osteosarcoma growth and spread. Inhibiting Cdc20 in osteosarcoma cells halts growth, induces apoptosis, and reduces metastasis, suggesting Cdc20 as a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Cell division cycle 20 homologue (Cdc20) is implicated in human tumorigenesis.
- The specific role of Cdc20 in osteosarcoma (OS) remains largely uninvestigated.
Purpose of the Study:
- To elucidate the function of Cdc20 in human osteosarcoma cells.
- To determine the impact of Cdc20 modulation on OS cell behavior and underlying molecular mechanisms.
Main Methods:
- Osteosarcoma cells were subjected to Cdc20 depletion or overexpression.
- Cell growth, apoptosis, cell cycle progression, migration, and invasion assays were performed.
- Western blotting was utilized to assess the expression of key proteins, including Bim and p21.
Main Results:
- Cdc20 down-regulation significantly inhibited OS cell growth, induced apoptosis, and caused cell cycle arrest.
- Reduced Cdc20 levels impaired OS cell migration and invasion capabilities.
- Conversely, Cdc20 overexpression promoted cell proliferation, suppressed apoptosis, and enhanced migration and invasion.
- Mechanistically, Cdc20 levels inversely correlated with the expression of Bim and p21 proteins.
Conclusions:
- Cdc20 plays a significant oncogenic role in osteosarcoma.
- Cdc20 promotes OS progression through the regulation of Bim and p21.
- Targeting Cdc20 presents a potential therapeutic strategy for osteosarcoma treatment.
Related Concept Videos
01:27Molecular Factors Affecting Cell Division
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
00:56What is the Cell Cycle?
01:04What is the Cell Cycle?
01:28The Cell Cycle Control System
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
02:11The Cell Cycle Control System
02:38Mitogens and the Cell Cycle