Related Experiment Video

Updated: Feb 21, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

The Arf-GAP and protein scaffold Cat1/Git1 as a multifaceted regulator of cancer progression

Sungsoo M Yoo1, Richard A Cerione2,3, Marc A Antonyak2

  • 1Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA.

Small Gtpases
|October 6, 2017
PubMed

Insights

Cool-associated tyrosine phosphorylated protein 1 (Cat1), also known as GPCR-kinase interacting protein 1 (Git1), regulates cell shape and migration. Emerging research suggests Cat1/Git1 may also play a role in oncogenic transformation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cat1/Git1 is a ubiquitously expressed, multi-domain protein involved in cell morphology and migration.
  • It functions as a GTPase activating protein (GAP) for ADP-ribosylation factor (Arf) GTPases.
  • Cat1/Git1 also acts as a scaffold protein, organizing signaling complexes within the cell.

Purpose of the Study:

  • To review the established roles of Cat1/Git1 in cell shape and migration.
  • To explore recent findings on Cat1/Git1's potential involvement in oncogenic transformation.

Main Methods:

  • Literature review of existing studies on Cat1/Git1.
  • Analysis of recent research data on Cat1/Git1 function.

Main Results:

  • Cat1/Git1 is confirmed to regulate cell morphology and migration through its GAP and scaffolding activities.
  • Preliminary evidence suggests Cat1/Git1 may contribute to cancer development through its signaling functions.

Conclusions:

  • Cat1/Git1 has a well-defined role in cellular dynamics.
  • Further investigation into Cat1/Git1's role in oncogenesis is warranted.
Keywords:
ArfCat1CoolGAPGit1Pixcancermigration

More Related Videos

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.5K
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

836

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.7K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.4K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K