Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
CRISPR01:59

CRISPR

58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
Homologous Recombination02:31

Homologous Recombination

64.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

pCODE tRNA Expression Plasmids Compensate for Rare Codons in Recombinant Protein Production.

ACS synthetic biology·2026
Same author

Aiolos and Eos drive distinct human TH17 functional states.

Cellular and molecular life sciences : CMLS·2026
Same author

Dissecting host stress responses for predictable heterologous gene expression in E. coli.

Nucleic acids research·2026
Same author

From Pancytopenia to Diagnosis: Visceral Leishmaniasis Identified Through Primary Care Assessment.

Cureus·2026
Same author

Language Barriers and Healthcare Challenges for Immigrants with Limited English Proficiency After Health Reform in the United States.

International journal of environmental research and public health·2026
Same author

Quality Control Technology for Abrasive Flow Precision Machining of a High-Performance Impeller.

Micromachines·2025

Related Experiment Video

Updated: Feb 21, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.6K

CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability.

Virginia Martínez1, Ida Lauritsen1, Tonja Hobel1

  • 1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.

Nucleic Acids Research
|October 6, 2017
PubMed
Summary

This study introduces a novel genome editing technique combining CRISPRi and the N-end rule to precisely control protein levels. This method offers enhanced control over gene expression and protein stability for various applications.

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.0K

Related Experiment Videos

Last Updated: Feb 21, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.6K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.0K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Gene interference is crucial for reverse genetics and cell manipulation.
  • Classical knockout and knockdown methods have limitations in controlling existing protein levels.

Purpose of the Study:

  • To develop an efficient genome editing approach for precise control of protein abundances.
  • To investigate the dependency of CRISPRi efficiency on endogenous gene expression levels.

Main Methods:

  • Utilized CRISPR interference (CRISPRi) for RNA synthesis control.
  • Integrated the N-end rule pathway to modulate protein degradation rates.
  • Developed a dual mechanism for fine-tuning protein levels.

Main Results:

  • Demonstrated a novel method to alter specific protein abundances by modulating both RNA synthesis and protein degradation.
  • Showcased that CRISPRi efficiency is influenced by endogenous gene expression levels.
  • Validated the approach for controlling protein levels in living cells.

Conclusions:

  • The developed genome editing approach offers precise control over protein abundances.
  • This method has broad applications in studying essential genes and discovering new antibiotics.
  • The findings advance the field of reverse genetics and biotechnological applications.