Adipose-derived stem cells attenuate pulmonary microvascular hyperpermeability after smoke inhalation
Koji Ihara1,2,3, Satoshi Fukuda1, Baigalmaa Enkhtaivan1
1Department of Anesthesiology, The University of Texas Medical Branch, Galveston, Texas, United States of America.
Plos One
|October 6, 2017
Summary
Adipose-derived stem cells (ASCs) infusion safely reduced lung fluid and improved gas exchange in a sheep model of acute respiratory distress syndrome (ARDS) induced by smoke inhalation.
Area of Science:
- Regenerative Medicine
- Pulmonary Medicine
- Critical Care
Background:
- Acute respiratory distress syndrome (ARDS) is a severe lung condition often caused by smoke inhalation, leading to high mortality in burn patients.
- Pulmonary edema is a key characteristic of ARDS.
- Adipose-derived stem cells (ASCs) possess significant anti-inflammatory properties.
Purpose of the Study:
- To evaluate the safety and efficacy of adipose-derived stem cells (ASCs) in a clinically relevant ovine model of ARDS.
- To determine if ASCs can mitigate the effects of smoke inhalation-induced ARDS.
Main Methods:
- ARDS was induced in female sheep via cooled cotton smoke inhalation.
- Sheep were ventilated and resuscitated, with cardiopulmonary hemodynamics monitored for 48 hours.
- Pulmonary microvascular hyper-permeability was assessed, and sheep were randomized to receive either ASCs or a control solution.
Main Results:
- ASC treatment significantly attenuated the increase in lung lymph flow and protein leakage associated with ARDS.
- ASCs nearly reversed increased pulmonary vascular permeability and lung water content.
- Pulmonary gas exchange improved significantly with ASC treatment, without adverse hemodynamic effects.
Conclusions:
- Intravenous administration of ASCs was well-tolerated in the ovine ARDS model.
- ASCs demonstrated the potential to modulate pulmonary microvascular hyper-permeability.
- The findings suggest ASCs may prevent the onset or progression of ARDS in this experimental setting.
