Related Experiment Video
Updated: Feb 21, 2026
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening
Published on: October 26, 2017
Plasma Exosomal miR-422a and miR-125b-2-3p Serve as Biomarkers for Ischemic Stroke
Dong-Bin Li1, Jing-Li Liu1, Wei Wang1
1Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Background:
MircroRNA (MiRNA) levels are associated with disease pathophysiology and are high in plasma exosomes. Plasma exosomal miRNAs serve as potential therapeutic targets and diagnosis biomarkers in some diseases but few studies have examined them in Ischemic Stroke (IS). Therefore, we explored the potential predictive value of plasma exosomal miR-422a and miR-125b-2-3p in different IS phases (acute and subacute phases).
Methods:
Fifty-five IS patients and 25 age and sex matched healthy controls were recruited. Patients were classified into two groups: 27 patients in acute phase (days 1-3) and 28 patients in subacute phase (days 4-14). The plasma exosomal levels of miR-422a and miR-125b-2-3p were examined via quantitative real-time polymerase chain reaction (qRT-PCR). The Areas Under the Curve (AUC) of the Receiver Operating Characteristic (ROC) curve were constructed to evaluate the diagnostic accuracy of these miRNAs in IS.
Results:
The expression levels of plasma exosomal miR-422a and miR-125b-2-3p were significantly decreased in the subacute phase group (P<0.001, P<0.001, respectively), and the miR-422a levels were increased in the acute phase group (P<0.005) as compared to the controls. Additionally, the expression levels of plasma exosomal miR-422a and miR-125b-2-3p were significantly decreased in the subacute phase group than in the acute phase group (P<0.001, P<0.005, respectively). ROC analysis showed high AUC values for miR-422a and miR-125b-2-3p in the subacute phase group as compared to those in healthy controls: 0.971 and 0.889, respectively, and miR-422a in the acute phase group as compared to healthy controls were 0.769.
Conclusion:
Plasma exosomal miR-422a and miR-125b-2-3p may serve as blood-based biomarkers for monitoring and diagnosing in IS patients, with plasma exosomal miR-422a showing the best diagnostic value. The use of these two plasma exosomal miRNAs in combination may be powerful for determining IS stage.
Insights
Plasma exosomal microRNAs (miRNAs) miR-422a and miR-125b-2-3p show potential as blood-based biomarkers for ischemic stroke (IS). miR-422a demonstrated the highest diagnostic value for IS, aiding in monitoring and diagnosis.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- MicroRNA (miRNA) dysregulation is implicated in disease pathophysiology.
- Plasma exosomal miRNAs are emerging biomarkers, but their role in Ischemic Stroke (IS) requires further investigation.
Purpose of the Study:
- To investigate the predictive value of plasma exosomal miR-422a and miR-125b-2-3p in acute and subacute phases of Ischemic Stroke (IS).
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure plasma exosomal miR-422a and miR-125b-2-3p levels in 55 IS patients and 25 healthy controls.
- Patients were categorized into acute (days 1-3) and subacute (days 4-14) phases.
- Receiver Operating Characteristic (ROC) curve analysis was performed to assess diagnostic accuracy.
Main Results:
- Plasma exosomal miR-422a and miR-125b-2-3p levels were significantly decreased in the subacute phase compared to controls (P<0.001).
- miR-422a levels were elevated in the acute phase compared to controls (P<0.005).
- ROC analysis revealed high diagnostic accuracy for miR-422a (AUC=0.971) and miR-125b-2-3p (AUC=0.889) in the subacute phase, and for miR-422a (AUC=0.769) in the acute phase.
Conclusions:
- Plasma exosomal miR-422a and miR-125b-2-3p can serve as blood-based biomarkers for IS monitoring and diagnosis.
- Plasma exosomal miR-422a exhibits the strongest diagnostic potential.
- Combining these miRNAs may enhance IS staging accuracy.
More Related Videos
Related Concept Videos
01:36Overview of Exosomes
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...