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Updated: Feb 21, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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Down-regulation of Inositol Polyphosphate 4-Phosphatase Type II Expression in Colorectal Carcinoma

Ji-Youn Sung1, Kiyong Na2, Hyun-Soo Kim3

  • 1Department of Pathology, Kyung Hee University School of Medicine, Seoul, Republic of Korea.

Anticancer Research
|October 7, 2017
PubMed
Abstract

Insights

Inositol polyphosphate 4-phosphatase type II (INPP4B) is significantly down-regulated in colorectal cancer (CRC) tissues and cell lines. This reduced expression of INPP4B suggests its involvement in CRC development and progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Aberrant survival signaling pathways drive cancer proliferation, apoptosis resistance, and metastasis.
  • Inositol polyphosphate 4-phosphatase type II (INPP4B) is a negative regulator of phosphatidylinositol 3-kinase signaling with known tumor-suppressive roles.

Purpose of the Study:

  • To investigate the expression levels of INPP4B in colorectal carcinoma (CRC).
  • To determine the role of INPP4B in the development and progression of CRC.

Main Methods:

  • Analysis of INPP4B mRNA and protein expression in CRC cell lines and patient tissues.
  • Techniques included Western blot, quantitative real-time reverse-transcriptase polymerase chain reaction, and immunohistochemical staining.

Main Results:

  • CRC cell lines exhibited significantly lower INPP4B protein and mRNA levels compared to normal colonic cells.
  • Reduced INPP4B expression was observed in 60.7% of primary and 76.5% of metastatic CRC tissues.
  • Normal colonic mucosa showed uniform, strong-to-moderate INPP4B immunoreactivity.

Conclusions:

  • INPP4B is demonstrably down-regulated in colorectal carcinoma.
  • The findings indicate that INPP4B plays a role in the pathogenesis of CRC.
Keywords:
Coloncolorectal carcinomaimmunohistochemistryinositol polyphosphate 4-phosphatase type IImetastasis

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