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Updated: Oct 23, 2026

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Detection of sequences that hybridize to human cytomegalovirus DNA in cervical neoplastic tissue
K Fletcher1, J W Cordiner, J C Macnab
1Institute of Virology, University of Glasgow.
Abstract:
Punch biopsies were taken from patients with cervical intraepithelial neoplasia and the DNA was extracted and examined for sequences which hybridized to human cytomegalovirus DNA by Southern blotting analysis. Two biopsies out of 43 were found to contain DNA which hybridized to human cytomegalovirus DNA. In one biopsy DNA sequences were detected which contained four restriction sites colinear with those of the prototype strain AD169. Evidence of rearranged viral DNA sequences was also found.

