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PKC-dependent phosphorylation of Munc18a at Ser313 in activated RBL-2H3 cells

Pratikshya Adhikari1, Hao Xu2

  • 1Department of Biological Sciences, University of Southern Mississippi, 118 College Drive, #5018, Hattiesburg, MS, 39406, USA.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|October 7, 2017
PubMed

Insights

Protein Kinase C (PKC) regulates mast cell degranulation. This study reveals PKC inhibition prevents Munc18a phosphorylation, suggesting a direct role in membrane fusion during inflammatory responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Mast cells release inflammatory compounds upon IgE/antigen activation.
  • Protein Kinase C (PKC) is implicated in regulating this process.
  • The precise mechanisms by which PKC controls mast cell degranulation remain unclear.

Purpose of the Study:

  • To elucidate the role of PKC in mast cell degranulation.
  • To investigate the molecular targets of PKC in this cellular process.
  • To determine if PKC directly influences membrane fusion machinery.

Main Methods:

  • Utilized the RBL-2H3 mast cell line.
  • Administered PKC inhibitor Ro-03-0432.
  • Assessed exocytosis/degranulation.
  • Analyzed Munc18a phosphorylation at Ser 313.

Main Results:

  • PKC inhibitor Ro-03-0432 demonstrated a concentration-dependent inhibition of RBL-2H3 cell exocytosis/degranulation.
  • The inhibitor prevented the phosphorylation of the membrane fusion factor Munc18a at the Ser 313 site.
  • This indicates a direct link between PKC activity and the regulation of key fusion proteins.

Conclusions:

  • PKC plays a significant role in regulating mast cell degranulation.
  • PKC-dependent phosphorylation of Munc18a is a key mechanism in controlling membrane fusion.
  • These findings offer novel insights into the molecular pathways governing inflammatory mediator release from mast cells.
Keywords:
InflammationMast cell degranulationMunc18aPhosphorylationProtein Kinase CRBL-2H3

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