Roseomonas terricola sp. nov., isolated from agricultural soil

A novel strain, designated EM302^T, was isolated from a soil sample collected from farmland located in Yesan-gun in the Republic of Korea. The cells were Gram-stain-negative, aerobic, non-motile without flagella and coccobacilli-shaped. Colonies were circular with entire edges, convex, opaque and pink. The strain grew at 15-40 ˚C (optimum, 35 ˚C) and pH 6.0-9.0 (optimum, 7.0). The strain grew in R2A medium without the addition of NaCl. Based on 16S rRNA gene sequences analysis, strain EM302^T was found to be phylogenetically related to Roseomonas wooponensis WW53^T (96.5 % 16S rRNA gene sequence similarity), Roseomonas arcticisoli MC 3624^T (95.2 %), Roseomonas aerilata 5420-30^T (94.5 %), Roseomonas pecuniae N75^T (94.4 %) and Roseomonas vinacea CPCC 100056^T (94.4 %). The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of the type strain was 65.7 mol%. On the basis of data presented here, strain EM302^T is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonasterricola sp. nov. is proposed. The type strain is EM302^T (=KACC 13942^T=KCTC 42906^T=NBRC 111477^T).