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Expression of matrix metalloproteinases-12 in ST-segment elevation myocardial infarction: A case-control study

Jing Wang1, Guoqing Wei, Wei Hu

  • 1Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, P.R. China.

Medicine
|October 7, 2017
PubMed

Insights

Matrix metalloproteinases-12 (MMP12) is present in coronary thrombus and elevated in ST-segment elevation myocardial infarction (STEMI) patients. This suggests MMP12 plays a role in STEMI, potentially linked to an imbalance with tissue inhibitor of metalloproteinases-1 (TIMP1).

Area of Science:

  • Cardiovascular Biology
  • Biochemistry
  • Molecular Medicine

Background:

  • Matrix metalloproteinases-12 (MMP12) degrades elastin, destabilizing plaques and promoting rupture.
  • MMP12 also influences platelet aggregation and secretion, relevant to thrombosis.
  • Limited research exists on MMP12's specific role in ST-segment elevation myocardial infarction (STEMI).

Purpose of the Study:

  • To investigate MMP12 expression in human coronary thrombus.
  • To examine the relationship between plasma MMP12 levels and STEMI.
  • To assess the balance between MMP12 and its inhibitor, TIMP1, in STEMI patients.

Main Methods:

  • Coronary thrombi and arterial plasma were collected from STEMI patients, stable angina pectoris (SAP) patients, and healthy controls.
  • MMP12 expression in thrombi was analyzed using immunohistochemistry, immunofluorescence, RT-PCR, Western blotting, and zymography.
  • Plasma MMP12 concentration (ELISA) and activity (FRET), along with TIMP1 concentration, were measured.

Main Results:

  • MMP12 was confirmed in human coronary thrombus, primarily as an active 45-kDa form.
  • STEMI patients exhibited significantly higher plasma MMP12 concentrations compared to SAP patients and controls.
  • Conversely, MMP12 activity was lower in STEMI patients, alongside elevated TIMP1 levels, indicating an MMP12/TIMP1 imbalance.

Conclusions:

  • MMP12 is present and active within human coronary thrombus.
  • Elevated plasma MMP12 concentrations and an altered MMP12/TIMP1 ratio are associated with STEMI.
  • These findings highlight MMP12 as a potential biomarker and therapeutic target in STEMI.

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