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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Feb 21, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

High-Throughput Approaches to Pinpoint Function within the Noncoding Genome.

Antonino Montalbano¹, Matthew C Canver², Neville E Sanjana¹

¹New York Genome Center, New York, NY, USA; Department of Biology, New York University, New York, NY, USA.

|October 7, 2017

Summary

This summary is machine-generated.

High-throughput CRISPR screens are revolutionizing the study of noncoding DNA, a previously under-explored genomic region. This review covers CRISPR-based methods for uncovering functional elements in noncoding DNA.

Keywords:

CRISPR Cas9 conservation enhancers functional genomics gene editing gene expression mutagenesis noncoding genome pooled screens

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Last Updated: Feb 21, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas nuclease system offers powerful genome editing capabilities.
CRISPR technology enables high-throughput genetic and epigenetic studies, extending functional interrogation to noncoding DNA.
Noncoding DNA has historically been less characterized than protein-coding genes due to limited perturbation methodologies.

Purpose of the Study:

To review high-throughput CRISPR-based approaches for identifying functional elements in the noncoding genome.
To discuss practical considerations for designing noncoding CRISPR libraries and analyzing screen data.

Main Methods:

Review of existing literature on CRISPR screens targeting noncoding genomic regions.
Discussion of CRISPR-Cas nuclease system applications in functional genomics.
Analysis of strategies for noncoding library design and experimental data interpretation.

Main Results:

An increasing number of CRISPR screens are emerging that specifically target noncoding genomic regions.
CRISPR technology provides a robust toolkit for perturbing and studying noncoding DNA.
These approaches facilitate the functional interrogation of previously under-characterized genomic elements.

Conclusions:

High-throughput CRISPR screens are crucial for advancing the understanding of noncoding DNA function.
Effective noncoding library design and analysis are key to successful CRISPR screens in this area.
This review provides a framework for researchers utilizing CRISPR technology to explore the noncoding genome.