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Updated: Jan 8, 2026

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

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Published on: June 13, 2020

Fluorescent protein Dendra2 as a ratiometric genetically encoded pH-sensor.

Alexey A Pakhomov¹, Vladimir I Martynov¹, Alexander N Orsa¹

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.

Biochemical and Biophysical Research Communications

|October 8, 2017

Summary

Dendra2 fluorescent protein can now serve as a ratiometric pH sensor. A new method using 350 nm excitation light enables accurate intracellular pH measurements in live cells.

Keywords:

Dendra2 Fluorescent proteins GFP Intracellular pH pH pH sensor

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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Area of Science:

Biochemistry
Cell Biology
Biophysics

Background:

Dendra2 is a photoconvertible fluorescent protein.
Its photoconverted form's fluorescence is pH-dependent.
Existing methods have reproducibility issues due to incomplete photoconversion.

Purpose of the Study:

To develop a reliable ratiometric pH sensor using Dendra2.
To establish a method for selective detection of photoconverted Dendra2.
To measure intracellular pH in live cells.

Main Methods:

Utilizing Dendra2's irreversible photoconversion from green to red emission.
Employing 350 nm excitation light to exclusively detect the photoconverted red form.
Measuring the red to green fluorescence ratio for ratiometric pH determination.

Main Results:

A method was developed to detect only the photoconverted Dendra2 fluorescence.
350 nm excitation selectively induced fluorescence from the photoconverted state.
Intracellular pH was successfully measured in CHO and HEK 293 cells.

Conclusions:

Dendra2 functions as a novel ratiometric genetically encoded pH sensor.
The developed method overcomes previous reproducibility limitations.
This sensor is suitable for live-cell imaging within the physiological pH range.