Related Experiment Video

Updated: Feb 21, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.5K

Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner

Alberto Brandariz-Núñez1, Fuxing Zeng1, Quan Ngoc Lam1

  • 1Department of Biochemistry, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, USA.

RNA (New York, N.Y.)
|October 8, 2017
PubMed

Insights

RNA-binding protein Sbp1 inhibits translation of Pab1 mRNA by binding its 5'UTR poly(A) sequence. Methylation of Sbp1

Area of Science:

  • Molecular Biology
  • RNA Metabolism
  • Gene Regulation

Background:

  • RNA-binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation.
  • Sbp1 is known to facilitate mRNA decapping but its mechanism of translation inhibition is unclear.
  • Pab1 is an essential translation factor with opposing roles in translation and decapping.

Purpose of the Study:

  • To elucidate the molecular mechanism of Sbp1-mediated translation inhibition.
  • To identify the specific interactions between Sbp1, Pab1 mRNA, and Pab1 protein.
  • To understand the role of Sbp1 in regulating Pab1 mRNA translation.

Main Methods:

  • In vitro binding assays to study RNA-protein and protein-protein interactions.
  • Analysis of mRNA translation in the presence of wild-type and modified Sbp1.
  • Site-directed mutagenesis to investigate the role of specific Sbp1 domains and modifications.

Main Results:

  • Sbp1 specifically and cooperatively binds the poly(A) sequence in the 5'UTR of Pab1 mRNA via its distal RRMs.
  • The central RGG domain of Sbp1 directly interacts with the Pab1 protein.
  • Methylation of arginines in the RGG domain disrupts Sbp1-Pab1 interaction and abolishes translation inhibition.
  • Sbp1 and its RGG repeats show distinct effects on transcript-specific translation.

Conclusions:

  • Sbp1 inhibits Pab1 mRNA translation initiation through direct interaction with both the Pab1 mRNA 5'UTR and the Pab1 protein.
  • RGG domain methylation is critical for Sbp1's inhibitory function.
  • RNA-binding proteins can regulate translation by remodeling the 5'UTR, impacting essential factors like Pab1.
Keywords:
5′UTR and polyadenosine-binding protein Pab1RNA-binding proteinSbp1translational regulation

More Related Videos

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.2K
Visualization of G3BP Stress Granules Dynamics in Live Primary Cells10:12

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

Published on: May 21, 2014

18.8K

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.8K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
701
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.8K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.6K
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
5.2K