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Related Concept Videos

Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K

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Updated: Feb 21, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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STAGE-Diging in Proteomics.

Paolo Soffientini1, Angela Bachi2

  • 1IFOM, FIRC Institute of Molecular Oncology, Via Adamello 16, 20139, Milan, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
PubMed
Summary
This summary is machine-generated.

A new STAGE-digestion method streamlines proteomics sample preparation, reducing handling time and improving protein identification. This technique enhances high-throughput proteomic analysis for clinical applications.

Keywords:
High-throughputIn-gel digestionMass spectrometryProteomicsSTAGE-digingSample processing

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biochemistry

Background:

  • Proteomics is a vital life science tool for analyzing protein abundance, modifications, and interactions.
  • Despite advancements in mass spectrometry, clinical proteomics applications are limited by time-consuming sample preparation.
  • Current methods face challenges in sample handling, recovery, and overall analysis time.

Purpose of the Study:

  • To introduce a modified in-gel digestion method, STAGE-digestion, to overcome current proteomics limitations.
  • To reduce sample handling and decrease analysis time in proteomic workflows.
  • To improve protein identification and quantification for high-throughput applications.

Main Methods:

  • A modified in-gel digestion protocol, termed STAGE-digestion, was developed.
  • The method focuses on reducing sample manipulation steps.
  • Efficiency was evaluated based on protein identification and quantification metrics.

Main Results:

  • STAGE-digestion significantly reduces sample handling and overall analysis time.
  • The modified method enhances protein identification and quantification accuracy.
  • Improved efficiency makes it suitable for labs with limited MS access or high-throughput needs.

Conclusions:

  • STAGE-digestion offers a more efficient and effective approach to proteomic sample preparation.
  • This method facilitates the integration of proteomics into routine clinical laboratory settings.
  • The technique supports high-throughput proteomic analyses within shorter timeframes.