Related Experiment Video
Updated: Feb 21, 2026
09:05
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
Published on: January 6, 2016
21.0K
Protein Arrays I: Antibody Arrays
Yulin Yuan1,2, Zuan-Tao Lin1, Hongting Wang1,3
1Department of Biomedical Engineering, University of Houston, 3605 Cullen Blvd, Houston, TX, 77204-5060, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
Summary
Antibody arrays, a protein detection platform, have advanced significantly with new technologies. This protocol details updated methods for antibody array development and application in protein analysis.
Area of Science:
- Biotechnology
- Proteomics
- Assay Development
Background:
- Antibody arrays are early protein array systems for high-throughput protein detection.
- Recent advancements in antibodies, probes, imaging, and computation have enhanced array performance.
- These improvements increase sensitivity, specificity, and robustness in protein analysis.
Purpose of the Study:
- To provide a comprehensive protocol incorporating the latest innovations in antibody array technology.
- To detail step-by-step experimental procedures for antibody array development and application.
- To highlight the utility of antibody arrays for various biological analyses.
Main Methods:
- Antibody printing techniques for array fabrication.
- Optimized sample preparation for enhanced target capture.
- Novel detection probes and imaging technologies for sensitive signal acquisition.
- Computational tools for robust data analysis.
Main Results:
- Demonstrated improvements in sensitivity and specificity of antibody arrays.
- Successful application of antibody arrays for cytokine profiling.
- Enabled mapping of post-translational modifications like phosphorylation and glycosylation.
Conclusions:
- Updated antibody array protocols offer a powerful tool for high-throughput protein analysis.
- The technology facilitates comprehensive profiling and modification mapping of proteins.
- Antibody arrays are crucial for advancing research in proteomics and biomarker discovery.
Related Concept Videos
02:34DNA Microarrays
21.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.4K
01:33Proteomics
9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
01:15Western Blotting
21.1K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
21.1K