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Protein Arrays I: Antibody Arrays

Yulin Yuan1,2, Zuan-Tao Lin1, Hongting Wang1,3

  • 1Department of Biomedical Engineering, University of Houston, 3605 Cullen Blvd, Houston, TX, 77204-5060, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
PubMed

Antibody arrays represent one of the very early protein array systems where antibodies are used to capture and detect target proteins in a high-throughput platform. The development of high-quality antibodies, nanomaterial-based novel detection probes, as well as innovative imaging technologies and computational tools has tremendously improved the sensitivity, specificity, and robustness of antibody arrays during the past decade. In this protocol we will incorporate the most updated innovations and developments of antibody arrays into the step-by-step experimental procedures. This includes antibody printing, sample preparation, array detection, as well as imaging and data analysis. Antibody array could be used for cytokine profiling or mapping of phosphorylation, glycosylation, or other post-translational modifications of target proteins.

Keywords:
Antibody arrayBiomarkersCytokine profilingGlycome mappingKinome mapping

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