Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

17.2K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.2K
Antibody Structure01:10

Antibody Structure

65.9K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.9K
Hybridoma Technology01:31

Hybridoma Technology

17.9K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.9K
Antibody Actions01:26

Antibody Actions

3.1K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular mechanisms of EPAS1 in regulating pulmonary vascular remodeling in COPD: a review.

Molecular biology reports·2026
Same author

The Role of <i>Trichoderma harzianum</i> Elicitor <i>Hyd1</i> in Inducing the Maize Endophytic Microbial Community and <i>Bacillus</i> Strains Against Maize Root Rot.

Journal of fungi (Basel, Switzerland)·2026
Same author

Safety Profile of COVID-19 Vaccines in HIV Patients Undergoing ART and Their Impact on Immune Recovery and HIV Reservoirs.

Infectious diseases & immunity·2026
Same author

Non-linear saturation threshold of gonadotropin dose on cumulative live birth rates in advanced-age women with polycystic ovary syndrome: a retrospective cohort study.

Frontiers in endocrinology·2026
Same author

<i>EPAS1</i> and <i>VEGFA</i> Polymorphisms Modulate COPD Susceptibility in a High-Altitude Population: A Case-Control Study.

International journal of chronic obstructive pulmonary disease·2026
Same author

<i>PPARA</i> Gene Polymorphisms and Their Association with COPD in Tibetan and Han Populations from Gansu, China.

International journal of chronic obstructive pulmonary disease·2026
Same journal

mRNA-LNP delivery of individual longevity genes mitigates doxorubicin-induced progeroid phenotypes.

Protein & cell·2026
Same journal

Targeting the TBK1-p62 condensate axis restores sensitivity to EGFR-TKIs in resistant lung cancer.

Protein & cell·2026
Same journal

Recent progresses in cancer multidrug resistance and therapeutic options associated with protein damage response.

Protein & cell·2026
Same journal

LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability.

Protein & cell·2026
Same journal

3D chromatin dynamics in cellular neuroendocrine transformation.

Protein & cell·2026
Same journal

Topology-Driven Activation of the GLP-1 Receptor Promotes Adipose Tissue Browning.

Protein & cell·2026
See all related articles

Related Experiment Video

Updated: Feb 21, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
04:47

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

8.2K

IgG Fc engineering to modulate antibody effector functions.

Xinhua Wang1, Mary Mathieu1, Randall J Brezski2

  • 1Genentech, Antibody Engineering, South San Francisco, CA, 94080, USA.

Protein & Cell
|October 8, 2017
PubMed
Summary
This summary is machine-generated.

Engineered therapeutic antibodies enhance immune responses for improved efficacy and safety. This review covers antibody engineering strategies, their mechanisms, and clinical applications of next-generation antibodies.

Keywords:
Fc engineeringFc gamma receptorantibody-dependent cell-mediated cytotoxicityantibody-dependent cellular phagocytosiscomplement-dependent cytotoxicitymonoclonal antibodyneonatal Fc receptor

More Related Videos

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
06:53

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

3.8K
Assessment of Human Natural Killer Cell Events Driven by Fc&#947;RIIIa Engagement in the Presence of Therapeutic Antibodies
09:54

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

3.9K

Related Experiment Videos

Last Updated: Feb 21, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
04:47

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

8.2K
Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
06:53

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

3.8K
Assessment of Human Natural Killer Cell Events Driven by Fc&#947;RIIIa Engagement in the Presence of Therapeutic Antibodies
09:54

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

3.9K

Area of Science:

  • Biotechnology
  • Immunology
  • Pharmacology

Background:

  • Therapeutic monoclonal antibodies are key biotherapeutics.
  • Human IgG1 subclass antibodies engage immune effector functions.
  • Molecular engineering allows modulation of antibody effector functions.

Purpose of the Study:

  • Review antibody engineering efforts to enhance efficacy and safety.
  • Discuss mechanisms of modified immune interactions.
  • Provide examples of next-generation antibody clinical studies.

Main Methods:

  • Literature review of antibody engineering strategies.
  • Analysis of proposed mechanisms for effector function modulation.
  • Compilation of clinical data on engineered antibodies.

Main Results:

  • Various engineering approaches exist to modify antibody effector functions.
  • Engineered antibodies show potential for improved therapeutic outcomes.
  • Next-generation antibodies are advancing in clinical trials.

Conclusions:

  • Antibody engineering offers a powerful tool to optimize therapeutic antibody performance.
  • Understanding immune interactions is crucial for designing effective next-generation antibodies.
  • Clinical studies demonstrate the promise of engineered antibodies in medicine.