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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

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Hemorrhagic Fever Virus Budding Studies.

Ronald N Harty¹

¹Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St., Philadelphia, PA, 19104, USA. rharty@vet.upenn.edu.

Methods in Molecular Biology (Clifton, N.J.)

|October 8, 2017

Summary

Researchers created virus-like particles (VLPs) from filovirus and arenavirus proteins to study virus budding. This safe, effective method aids in discovering inhibitors for virus release and spread.

Keywords:

Budding Ebola virus Matrix protein TIRF VLPs VP40

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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Area of Science:

Virology
Cell Biology
Drug Discovery

Background:

Filoviruses (e.g., Marburg, Ebola) and arenaviruses (e.g., Lassa, Junín) cause significant disease.
Virus budding is a critical step in the replication and spread of many viruses.
Understanding the mechanisms of virus budding is essential for developing antiviral therapies.

Purpose of the Study:

To establish a safe and reliable assay for studying the budding process of filoviruses and arenaviruses.
To investigate the role of matrix proteins VP40 and Z in virus-like particle (VLP) production and release.
To validate the VLP budding assay as a tool for identifying small molecule inhibitors of viral release.

Main Methods:

Independent expression of VP40 (filoviruses) and Z (arenaviruses) matrix proteins in mammalian cells.
Production and release of virus-like particles (VLPs) morphologically identical to infectious virions.
Quantification of VLP production and egress using techniques including SDS-PAGE, Western blotting, and total internal reflection fluorescence (TIRF) microscopy.

Main Results:

Transient transfection successfully produced VLPs from filovirus and arenavirus matrix proteins.
VLP production and release were effectively detected and quantified using established biochemical and imaging methods.
The VLP budding assay accurately mimicked the budding of infectious viruses.

Conclusions:

The VLP budding assay is a safe (BSL-2) and effective method for studying viral budding.
This assay can be used to identify and validate host and viral factors involved in virus release.
The assay serves as a valuable screening platform for discovering small molecule inhibitors of virus spread.