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Quantification of NMDAR Subunit Genes Expression by qRT-PCR
1Molecular and Cellular Biology Platform, INSERM U901, Mediterranean Institute of Neurobiology (INMED), Aix-Marseille University, Marseille, France. emilie.pallesi@inserm.fr.
Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
Summary
This study details quantifying NMDAR subunit transcription during brain development and disease. We utilize reverse-transcription quantitative polymerase chain reaction (RT-qPCR) for accurate gene expression analysis.
Area of Science:
- Neuroscience
- Molecular Biology
- Gene Expression Analysis
Background:
- Transcription is a critical gene expression control point.
- Cellular needs and environmental cues heavily influence transcription.
- NMDAR subunits play vital roles in brain function.
Purpose of the Study:
- To present a method for quantifying NMDAR subunit transcriptional expression.
- To analyze NMDAR expression during brain development.
- To investigate NMDAR expression in healthy and pathological conditions.
Main Methods:
- Utilizing reverse-transcription quantitative polymerase chain reaction (RT-qPCR).
- Quantifying mRNA levels of specific NMDAR subunits.
- Applying the method to developmental and disease models.
Main Results:
- RT-qPCR provides a sensitive measure of NMDAR subunit transcription.
- Expression patterns of NMDAR subunits vary during development.
- Altered NMDAR expression is observed in pathological states.
Conclusions:
- RT-qPCR is a robust technique for studying NMDAR gene expression.
- Understanding NMDAR expression is crucial for brain development and disease.
- This quantification method aids in neurological research.
