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Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
Luigi Mele1, Davide Liccardo1, Virginia Tirino2
1Department of Experimental Medicine, Medical School, University of Campania "Luigi Vanvitelli", Naples, Italy.
Abstract:
The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids. In this chapter, it is described the role of ALDH in the identification of cancer stem cells (CSCs), having been shown that stem cells express high levels of ALDH. Here, we present a method called ALDEFLUOR assay used for the identification, evaluation, and isolation of normal, cancer stem and progenitor cells.

