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A validated method for analyzing polyunsaturated free fatty acids from dried blood spots using LC-MS/MS
Erandi Hewawasam1, Ge Liu1, David W Jeffery1
1FOODplus Research Centre, School of Agriculture, Food and Wine, the University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia.
Prostaglandins, Leukotrienes, and Essential Fatty Acids
|October 9, 2017
Summary
A new liquid chromatography tandem mass spectrometry method accurately quantifies omega-3 and omega-6 polyunsaturated free fatty acids (PUFA-FFA) in dried blood spots. This sensitive and precise technique offers a reliable way to measure these key inflammation regulators.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Omega-3 and omega-6 polyunsaturated free fatty acids (PUFA-FFA) are crucial precursors for lipid mediators that regulate inflammation.
- Accurate quantification of individual PUFA-FFA is essential for understanding inflammatory processes.
Purpose of the Study:
- To develop and validate a novel, sensitive method for quantifying individual PUFA-FFA in dried blood spots.
- To establish a precise analytical technique for measuring PUFA-FFA relevant to inflammation.
Main Methods:
- Development of a liquid chromatography tandem mass spectrometry (LC-MS/MS) method.
- Lipid extraction from dried blood spots followed by LC-MS/MS quantification using stable isotope dilution analysis.
- Validation of the method by comparing results with traditional thin layer chromatography-gas chromatography (TLC-GC) methods.
Main Results:
- The LC-MS/MS method demonstrated linearity across expected clinical concentration ranges.
- Intra-day and inter-day variations for all quantified PUFA-FFA were ≤ 16%.
- LC-MS/MS measurements showed positive correlations with traditional TLC-GC method results.
Conclusions:
- A novel, sensitive, and precise LC-MS/MS method for PUFA-FFA quantification in dried blood spots has been successfully developed and validated.
- This method accurately measures biologically important PUFA-FFA levels in blood, offering a valuable tool for research and clinical applications.
- The dried blood spot approach simplifies sample collection and handling for PUFA-FFA analysis.
