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MiR-206 inhibits Head and neck squamous cell carcinoma cell progression by targeting HDAC6 via PTEN/AKT/mTOR pathway

Fangzhou Liu1, Xiaotong Zhao2, Yichun Qian1

  • 1Department of Head and Neck Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nangjing 210000, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|October 9, 2017
PubMed

Insights

MicroRNA-206 (miR-206) is decreased in Head and Neck Squamous Cell Carcinoma (HNSCC). Restoring miR-206 inhibits HNSCC cell proliferation, migration, and invasion by targeting HDAC6, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) are endogenous noncoding small RNAs regulating physiological functions.
  • The role of miRNAs in Head and Neck Squamous Cell Carcinoma (HNSCC) senescence remains largely unknown.
  • This study investigates the function of miR-206 in HNSCC.

Purpose of the Study:

  • To determine the expression level of miR-206 in HNSCC tissues and cell lines.
  • To explore the biological functions of miR-206 in HNSCC progression, including proliferation, migration, and invasion.
  • To elucidate the molecular mechanism by which miR-206 exerts its effects in HNSCC.

Main Methods:

  • Real-time quantitative PCR (qPCR) to measure miR-206 expression.
  • MTT assay, colony formation, and cell cycle analysis to assess proliferation.
  • Wound healing and Transwell assays to evaluate migration and invasion.
  • Luciferase reporter assays to confirm targeting of HDAC6.
  • Western blot to analyze signaling pathway proteins (PTEN/AKT/mTOR).

Main Results:

  • miR-206 expression was significantly decreased in HNSCC tissues and cell lines compared to normal controls.
  • Restoration of miR-206 inhibited HNSCC cell proliferation, migration, and invasion.
  • miR-206 induced S-phase cell cycle arrest in HNSCC cells.
  • miR-206 directly targets the 3'UTR of HDAC6.
  • miR-206 inhibits HNSCC progression by targeting HDAC6 through the PTEN/AKT/mTOR pathway.

Conclusions:

  • miR-206 plays a crucial role in HNSCC progression.
  • miR-206 acts as a tumor suppressor in HNSCC by targeting HDAC6 via the PTEN/AKT/mTOR pathway.
  • miR-206 represents a potential diagnostic and therapeutic target for HNSCC.
Keywords:
HDAC6HNSCCMiR-206PTEN/AKT/mTOR pathwayProgression

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