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Placenta-specific gene manipulation using lentiviral vector and its application.

Tomohiro Tobita1, Daiji Kiyozumi2, Masahito Ikawa1

  • 1Research Institute for Microbial Diseases, Osaka University, Osaka, Japan; Graduate School of Medicine, Osaka University, Osaka, Japan.

Placenta
|October 10, 2017
PubMed
Summary

Lentiviral (LV) vectors enable placenta-specific gene modification in mammals. This technology allows researchers to study placental development by targeting the placenta without altering the fetal genome.

Keywords:
CRISPR/Cas9 systemGene therapyGenome editingPlacentaPreeclampsiaViral vectors

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • The placenta is crucial for mammalian embryonic development.
  • Studying placental function is challenging due to the intertwined placental and fetal genomes in genetically modified animals.
  • A method to modify the placental genome independently of the fetal genome is needed.

Purpose of the Study:

  • To review the development and applications of lentiviral (LV) vector-mediated placenta-specific gene manipulation.
  • To discuss the potential of LV vectors in advancing placental research.

Main Methods:

  • Lentiviral (LV) vectors are used for gene transfer.
  • Transducing embryos at the blastocyst stage leads to trophectoderm-specific viral transduction.
  • This results in transgene expression specifically within the placenta.

Main Results:

  • LV vectors can be used to achieve placenta-specific gene modification.
  • This technology overcomes the challenge of distinguishing placental defects from fetal effects.
  • Over a decade of research has utilized this method for placental studies.

Conclusions:

  • LV vector-mediated placenta-specific gene manipulation is a powerful tool for placental research.
  • Future applications may involve combining LV vectors with genome editing technologies for deeper insights into placental function.