Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

753
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
753
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

600
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
600
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.3K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved Extracellular Level of L-Asparaginase in Shaken Cultures of Escherichia coli ATCC 11,303 Using a Statistical Design Approach.

Applied biochemistry and biotechnology·2026
Same author

Enhanced anti-EpCAM ScFv production and reduced acetate overflow in a BW25113-derived E. coli strain with ArcA and Pka deletions.

Microbial cell factories·2025
Same author

Optimizing Gene Sources for L-asparaginase Production: A Comparative Review.

Current microbiology·2025
Same author

Cisplatin-loaded UiO-66-NH<sub>2</sub> functionalized with folic acid enhances apoptotic activity and antiproliferative effects in MDA-MB-231 breast and A2780 ovarian cancer cells: An <i>in vitro</i> study.

Heliyon·2025
Same author

CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.

BioImpacts : BI·2025
Same author

Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line.

Research in pharmaceutical sciences·2025

Related Experiment Video

Updated: Feb 21, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

11.4K

Multiple-locus variable-number tandem-repeat analysis in Salmonella isolates as an effective molecular subtyping

Atieh Hashemi1, Fahimeh Baghbani-Arani2, Sepideh Ahmadiyan3

  • 1Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Microbial Pathogenesis
|October 10, 2017
PubMed
Summary

Multiple Loci Variable Number of Tandem Repeats (VNTR) analysis (MLVA) effectively differentiates Salmonella enterica isolates. This molecular genotyping method shows high discriminatory power for tracking bacterial strains in Iran.

Keywords:
MLVAMolecular typingSalmonellaSerovarVNTR

More Related Videos

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

22.4K
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.2K

Related Experiment Videos

Last Updated: Feb 21, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

11.4K
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

22.4K
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.2K

Area of Science:

  • Microbiology
  • Molecular Epidemiology
  • Genetics

Background:

  • Traditional serotyping methods have limitations in differentiating closely related microbial isolates and investigating disease outbreaks.
  • Multiple Loci Variable Number of Tandem Repeats (VNTR) analysis (MLVA) has emerged as a powerful molecular genotyping technique.
  • Previous studies have shown the utility of MLVA for specific Salmonella serovars in Iran.

Purpose of the Study:

  • To evaluate the discriminatory ability of MLVA for molecular typing of Salmonella enterica isolates from various human sources in Iran.
  • To assess the effectiveness of specific VNTR loci and their combinations in differentiating Salmonella strains.
  • To determine the suitability of MLVA for the molecular epidemiology of Salmonella.

Main Methods:

  • Seventy-eight Iranian Salmonella enterica isolates from human clinical samples (urine, stool, bone marrow, blood, ascites, synovial fluid) collected between 2012 and 2015 were analyzed.
  • Six VNTR loci were amplified from all isolates.
  • Genotyping was performed using MLVA, and discriminatory indices (DI) were calculated for individual markers and combined loci.

Main Results:

  • The 78 Salmonella isolates, comprising 70 typed and 8 nontypeable strains across eight serotypes/serogroups, were distributed into 67 distinct genotypes.
  • Two specific VNTR markers, Sal20 and Sal16, demonstrated high discriminatory ability (DI > 0.80) for all strains.
  • The combined analysis of all six VNTR loci yielded 67 different types with a cumulative discriminatory value (D) of 0.995.

Conclusions:

  • MLVA exhibits a high discriminatory power for molecular typing of Salmonella enterica.
  • The combination of multiple VNTR loci significantly enhances the ability to differentiate Salmonella strains.
  • MLVA is a valuable tool for the molecular epidemiology of Salmonella, aiding in outbreak investigations and strain characterization.