Immunoneutralization of somatostatin and neurotensin: effect on gastric acid secretion

Cell lines producing monoclonal antibodies to somatostatin and neurotensin, designated S-10 and NT-C5, respectively, have recently been generated. The purpose of the present immunoneutralization study in urethan-anesthetized gastric fistula rats is 1) to examine the ability of these antibodies to block the inhibitory effect of their target peptides on meal-stimulated gastric acid secretion and 2) to use these antibodies as probes to determine whether somatostatin and/or neurotensin are involved in the inhibition of gastric acid secretion produced by intraduodenal, intra-ileal, and intracolonic fat infusions. The results demonstrate that both S-10 and NT-C5 successfully bound exogenous somatostatin and neurotensin in vivo. S-10 but not NT-C5 prevented the inhibition of gastric acid secretion produced by intraduodenal fat. NT-C5 but not S-10 prevented the inhibition of gastric acid secretion produced by intra-ileal fat. Neither S-10 nor NT-C5 prevented the inhibition of gastric acid secretion produced by intracolonic fat. We conclude that somatostatin is associated with proximal and neurotensin with distal small bowel intestinal fat-induced inhibition of gastric acid secretion.