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Alanine aminopeptidase in serum: biological variations and reference limits
G J Sanderink1, Y Artur, F Schiele
1Laboratory Department (UA CNRS No 597), Vandoeuvre-lès-Nancy, France.
Clinical Chemistry
|July 1, 1988
Summary
Serum alanine aminopeptidase (AAP) levels vary significantly with age, sex, smoking, alcohol consumption, and certain medications. These factors are crucial for establishing accurate reference ranges for AAP in clinical practice.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Human Physiology
Background:
- Alanine aminopeptidase (AAP) is an enzyme found in human serum.
- Understanding biological variations in AAP is essential for accurate clinical interpretation.
Purpose of the Study:
- To investigate the influence of demographic, lifestyle, and pharmacological factors on serum AAP activity.
- To establish reliable reference limits for serum AAP.
Main Methods:
- Automated assay using alanyl-4-nitroanilide as substrate.
- Analysis of serum samples from 2178 apparently healthy individuals and subjects on specific medications.
Main Results:
- Serum AAP levels were higher in males than females across most age groups.
- Highest AAP values observed in children (10-14 years).
- Smoking, high ethanol intake, and oral contraceptives increased AAP; hypolipidemic drugs decreased it.
- Anticonvulsant drugs correlated with higher AAP activity.
Conclusions:
- Demographic, lifestyle, and drug interactions significantly impact serum AAP levels.
- Established reference limits for AAP, considering identified influencing factors.
Keywords:
Age FactorsAlcohol DrinkingBehaviorBiologyContraceptionContraceptive MethodsDemographic FactorsDeveloped CountriesDiseasesEnzymesEnzymes And Enzyme InhibitorsEuropeFamily PlanningFranceHemic SystemHepatic EffectsHypertensionMediterranean CountriesOral ContraceptivesPhysiologyPopulationPopulation CharacteristicsSex FactorsSmokingVascular DiseasesWestern Europe